DETECTION OF GENE ENHANCERS BY Drosophila ENHANCER TRAP ANALYSIS Enhancers: Transcriptional enhancers are DNA sequences
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چکیده
Enhancers: Transcriptional enhancers are DNA sequences that control expression of nearby genes and direct tissue-specific, positive regulation of those genes (Karp, 2002; Griffiths, et al., 2002). The general mode of action of these sequences is that they associate with specific activator proteins (that are found in only certain tissues); this association of enhancer and activator facilitates transcription initiation at the target gene's promoter. Often located 1 kb or more from the target promoter, enhancers might be located upstream, downstream or even within the transcription unit that they control. Under the influence of one or more enhancers, a gene can be abundantly expressed in certain tissues of the organism (i.e., cells in which the activator protein is found) and weakly or not at all expressed in other tissues. By identifying and studying enhancers, we learn how genes are regulated in time and space within multicellular organisms and we are able to use enhancers to "tag" tissues or even individual cells.
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DETECTION OF GENE ENHANCERS BY Drosophila ENHANCER TRAP ANALYSIS
Enhancers: Transcriptional enhancers are DNA sequences that control expression of nearby genes and direct tissue-specific, positive regulation of those genes (Walker & Rapley,1997; also, any contemporary genetics or cell biology textbook). The general mode of action of these sequences is that they associate with specific activator proteins (that are found in only certain tissues); this associat...
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